Choosing the Right HPLC Column Supplier

The hplc column supplier is one of the most important pieces of equipment in liquid chromatography (LC). They are used for the separation of different components in a sample and can be based on the type of mobile phase, stationary phase, pore size, affinity or ion charges.

The HPLC (high-performance liquid chromatography) technique was developed in the 1960s and is now widely used for many chemical analysis applications, including biomedical research, environmental sciences and pharmaceutical industries. The process involves a sample with a suitable solvent (mobile phase) being injected into a LC column filled with packed particles. Then, the separated compounds are eluted from the column at different times, so that they can be put to use or analyzed for their compositions and properties.

A typical HPLC column is made of glass or metal and contains normal phase or reverse phase stationary phases, which are based on silica gel, polymer gels or calcium carbonate. The mobile phase consists of an organic solvent, which is usually water.

There are many types of chromatography columns available in the market today, and it is important to choose the right one for your application. In addition to selecting the type of HPLC column, you should also consider factors such as the size and number of beads in the column, the length of the column, the column internal diameter and the method.

Nano HPLC is a form of HPLC that uses columns with very small inner diameters. This reduces the required injection and flow rates, which makes it particularly beneficial when only small or diluted sample quantities are available.

These small inner diameters allow for a high sensitivity with reduced solvent consumption. In addition, they enable a better resolution and efficiency.

Ultra-High Performance Liquid Chromatography is a special chromatography technique that separates the sample by using very small particles in the mobile phase, allowing the sample to be resolved to a finer resolution than traditional LC. This technique is often used to separate compounds such as proteins, carbohydrates and amino acids.

UPLC is the industry’s leading modern technology for HPLC columns and has more than 200 configurations with different chemistries. The wide range of UPLC columns from Waters offer faster separations with superior resolution and sensitivities, which can help increase your productivity while meeting your analytical challenges.

The UPLC system allows you to control and monitor multiple UPLC columns simultaneously, enabling you to achieve the highest level of productivity while optimizing your lab workflows. This allows you to quickly and accurately analyze your samples for more accurate results while improving your bottom line.

When selecting a UPLC column, ensure that the internal diameter of the column is not too narrow or too large, as this can negatively impact your resolution and efficiency. This could be a risk for analytical applications with high backpressure, and it is recommended to select a column with a diameter that matches the desired analytical objectives.